Genetic Engineering: A Powerful Tool for Crop Improvement

Rising population, changing climatic conditions, and various biotic and abiotic stresses are contributors to lowering crop yields. This, in turn, has augmented the number of people suffering from malnutrition. The applications of genetic engineering including genome editing are important as it can complement modern breeding activities to mitigate the effects of changing environment and boost crop production. The genetically modified (GM) crops thus offer one or more advantageous attributes, such as herbicide resistance, tolerance against pests and pathogens, and nutritional enhancement. The discovery of the natural ability of Agrobacterium tumefaciens to transfer a segment of its DNA (T-DNA) into the host was one of the breakthroughs of the twentieth century. It marked the beginning of achieving successful genetic transformation in a wide range of plants. Further, with the advent of technologies like zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas, it has been possible to overcome the limitations of conventional breeding techniques. The synergism of scientific skills with sophisticated technologies resulted in many successful GM crops that were resistant to insects, pests, and weeds and enriched in micronutrients like vitamins and various minerals. Although not all GM crops have been commercialized, a few like soybean, papaya, maize, cotton, common bean, sweet potato, cowpea, etc. are practising. Recently, genome-edited crops are also approved for commercialization. The technology holds immense promise to achieve UN’s sustainable development goals (SDGs) to fight hunger, attain food security, enhance nutrition, and promote sustainable agriculture.

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References

• Abbas MA, Haroun SA, Mowafy AM (2021) Dual inoculation of Bradyrhizobium and Enterobacter alleviates the adverse effect of salinity on Glycine max seedling. Not Bot Horti Agrobot Cluj-Napoca 49(3):12461–12461 ArticleGoogle Scholar
• Ahmar S, Saeed S, Khan MHU, Ullah Khan S, Mora-Poblete F, Kamran M, Jung KH (2020) A revolution toward gene-editing technology and its application to crop improvement. Int J Mol Sci 21(16):5665 ArticleCASPubMedPubMed CentralGoogle Scholar
• Al Atalah B, Smagghe G, Van Damme EJ (2014) Orysata, a jacalin-related lectin from rice, could protect plants against biting-chewing and piercing-sucking insects. Plant Sci 221:21–28 ArticlePubMedGoogle Scholar
• Azadi H, Samiee A, Mahmoudi H, Jouzi Z, Rafiaani Khachak P, De Maeyer P, Witlox F (2016) Genetically modified crops and small-scale farmers: Main opportunities and challenges. Crit Rev Biotechnol 36:434–446 PubMedGoogle Scholar
• Barrangou R, Doudna JA (2016) Applications of CRISPR technologies in research and beyond. Nat Biotechnol 34(9):933–941 ArticleCASPubMedGoogle Scholar
• Barry GF, Kishore GM, Padgette SR, Stallings WC (1997) Glyphosate-Tolerant 5-Enolpyruvylshikimate-3-Phosphate Synthases. U.S. Patent No 5,633,435. U.S. Patent and Trademark Office, Washington, DC Google Scholar
• Baum JA, Bogaert T, Clinton W, Heck GR, Feldmann P, Ilagan O, Roberts J (2007) Control of coleopteran insect pests through RNA interference. Nat Biotechnol 25(11):1322–1326 ArticleCASPubMedGoogle Scholar
• Bhatia S, Bera T, Dahiya R, Bera T, Bhatia S, Bera T (2015) Classical and nonclassical techniques for secondary metabolite production in plant cell culture. In: Modern applications of plant biotechnology in pharmaceutical sciences, pp 231–291 ChapterGoogle Scholar
• Boston RS, Viitanen PV, Vierling E (1996) Molecular chaperones and protein folding in plants. Plant Mol Biol 32(1–2):191–222 ArticleCASPubMedGoogle Scholar
• Botterman J, Leemans J (1988) Engineering of herbicide resistance in plants. Biotechnol Genet Eng Rev 6(1):321–340 ArticleCASGoogle Scholar
• Broadway RM, Duffey SS (1986) The effect of dietary protein on the growth and digestive physiology of larval Heliothis zea and Spodoptera exigua. J Insect Physiol 32(8):673–680 ArticleCASGoogle Scholar
• Bruening G, Lyons J (2000) The case of the FLAVR SAVR tomato. Calif Agr 54(4):6–7 ArticleGoogle Scholar
• Cantos C, Francisco P, Trijatmiko KR, Slamet-Loedin I, Chadha-Mohanty PK (2014) Identification of “safe harbor” loci in indica rice genome by harnessing the property of zinc-finger nucleases to induce DNA damage and repair. Front Plant Sci 5:1–8 ArticleGoogle Scholar
• Carlson DF, Fahrenkrug SC, Hackett PB (2012) Targeting DNA with fingers and TALENs. Mol Ther–Nucleic Acids 1:e3. https://doi.org/10.1038/mtna.2011.5ArticleCASPubMedPubMed CentralGoogle Scholar
• Carroll D, Morton JJ, Beumer KJ, Segal DJ (2006) Design, construction and in vitro testing of zinc finger nucleases. Nat Protoc 1(3):1329–1341 ArticleCASPubMedGoogle Scholar
• Castiglioni P, Warner D, Bensen RJ, Anstrom DC, Harrison J, Stoecker M, Heard JE (2008) Bacterial RNA chaperones confer abiotic stress tolerance in plants and improved grain yield in maize under water-limited conditions. Plant Physiol 147(2):446–455 ArticleCASPubMedPubMed CentralGoogle Scholar
• Chaitanya KV, Sundar D, Masilamani S, Ramachandra Reddy A (2002) Variation in heat stress-induced antioxidant enzyme activities among three mulberry cultivars. Plant Growth Regul 36(2):175–180 ArticleCASGoogle Scholar
• Chakroun M, Banyuls N, Bel Y, Escriche B, Ferré J (2016) Bacterial vegetative insecticidal proteins (Vip) from entomopathogenic bacteria. Microbiol Mol Biol Rev 80(2):329–350 ArticleCASPubMedPubMed CentralGoogle Scholar
• Chen H, Xiong L (2009) Enhancement of vitamin B6 levels in seeds through metabolic engineering. Plant Biotechnol J 7(7):673–681 ArticlePubMedGoogle Scholar
• Chen TH, Murata N (2002) Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Curr Opin Plant Biol 5(3):250–257 ArticleCASPubMedGoogle Scholar
• Cheung AY, Bogorad L, Van Montagu M, Schell J (1988) Relocating a gene for herbicide tolerance: a chloroplast gene is converted into a nuclear gene. Proc Natl Acad Sci U S A 85(2):391–395 ArticleCASPubMedPubMed CentralGoogle Scholar
• Chilton MD, Drummond MH, Merio DJ, Sciaky D, Montoya AL, Gordon MP, Nester EW (1977) Stable incorporation of plasmid DNA into higher plant cells: the molecular bases of crown gall tumorigenesis. Cell 11:263–271 ArticleCASPubMedGoogle Scholar
• Chrispeels MJ, Sadava DE (2003) Plants, genes, and crop biotechnology. Jones and Bartlett Publisher. SN–9780763715861 Google Scholar
• Clasen BM, Stoddard TJ, Luo S, Demorest ZL, Li J, Cedrone F, Tibebu R, Davison S, Ray EE, Daulhac A, Coffman A, Yabandith A, Retterath A, Haun W, Baltes NJ, Mathis L, Voytas DF, Zhang F (2016) Improving cold storage and processing traits in potato through targeted gene knockout. Plant Biotechnol J 14(1):169–176 ArticleCASPubMedGoogle Scholar
• Datta K, Baisakh N, Oliva N, Torrizo L, Abrigo E, Tan J, Rai M, Rehana S, Al-Babili S, Beyer P, Potrykus I, Datta SK (2003) Bioengineered ‘golden’ indica rice cultivars with β-carotene metabolism in the endosperm with hygromycin and mannose selection systems. Plant Biotechnol J 1:81–90 ArticleCASPubMedGoogle Scholar
• Davuluri GR, Van Tuinen A, Fraser PD, Manfredonia A, Newman R, Burgess D, Bowler C (2005) Fruit-specific RNAi-mediated suppression of DET1 enhances carotenoid and flavonoid content in tomatoes. Nat Biotechnol 23(7):890–895 ArticleCASPubMedGoogle Scholar
• Demorest ZL, Coffman A, Baltes NJ, Stoddard TJ, Clasen BM, Luo S et al (2016) Direct stacking of sequence-specific nuclease-induced mutations to produce high oleic and low linolenic soybean oil. BMC Plant Biol 16:1–8 ArticleGoogle Scholar
• Deng LH, Weng LS, Xiao GY (2014) Optimization of Epsps gene and Development of double herbicide tolerant transgenic PGMS Rice. J Agric Sci Technol 16:217–228 CASGoogle Scholar
• Deutsch CA, Tewksbury JJ, Huey RB, Sheldon KS, Ghalambor CK, Haak DC, Martin PR (2008) Impacts of climate warming on terrestrial ectotherms across latitude. Proc Natl Acad Sci 105(18):6668–6672 ArticleCASPubMedPubMed CentralGoogle Scholar
• Dianov GL, Hübscher U (2013) Mammalian base excision repair: the forgotten archangel. Nucleic Acids Res 41(6):3483–3490 ArticleCASPubMedPubMed CentralGoogle Scholar
• Dively GP, Kuhar TP, Taylor S, Doughty HB, Holmstrom K, Gilrein D et al (2021) Sweet corn sentinel monitoring for lepidopteran field-evolved resistance to Bt toxins. J Econ Entomol 114:307–319 ArticleCASPubMedGoogle Scholar
• Duan X, Li X, Xue Q, Abo-EI-Saad M, Xu D, Wu R (1996) Transgenic rice plants harboring an introduced potato proteinase inhibitor II gene are insect resistant. Nat Biotechnol 14(4):494–498 ArticleCASPubMedGoogle Scholar
• Dubock A (2019) Golden rice: to combat vitamin a deficiency for public health. Vitamin A:1–21 Google Scholar
• Dunse KM, Stevens JA, Lay FT, Gaspar YM, Heath RL, Anderson MA (2010) Coexpression of potato type I and II proteinase inhibitors gives cotton plants protection against insect damage in the field. Proc Natl Acad Sci 107(34):15011–15015 ArticleCASPubMedPubMed CentralGoogle Scholar
• Durai S, Mani M, Kandavelou K, Wu J, Porteus MH, Chandrasegaran S (2005) Zinc finger nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells. Nucleic Acids Res 33:5978–5990 ArticleCASPubMedPubMed CentralGoogle Scholar
• FAO (2020) The state of world fisheries and aquaculture. Sustainability in action. Rome Google Scholar
• Faria JC, Albino MMC, Dias BBA, Cançado LJ, da Cunha NB, de Silva ML, Aragão FJL (2006) Partial resistance to bean golden mosaic virus in a transgenic common bean (Phaseolus vulgaris L.) line expressing a mutated rep gene. Plant Sci 171(5):565–571 ArticleCASGoogle Scholar
• Ferreira SA, Pitz KY, Manshardt R, Zee F, Fitch M, Gonsalves D (2002) Virus coat protein transgenic papaya provides practical control of papaya ring-spot virus in Hawaii. Plant Dis 86:101–105 ArticlePubMedGoogle Scholar
• Fitch MMM, Manshardt RM, Gonsalves D, Slightom JL, Sanford JC (1992) Virus resistant papaya derived from tissues bombarded with the coat protein gene of papaya ringspot virus. Biotechnol 10:1466–1472 CASGoogle Scholar
• Flores-Mireles AL, Eberhard A, Winans SC (2012) Agrobacterium tumefaciens can obtain Sulphur from an opine that is synthesized by octopine synthase using S-methylmethionine as a substrate. Mol Microbiol 84(5):845–856 ArticleCASPubMedPubMed CentralGoogle Scholar
• Fraley RT, Rogers SG, Horsch RB, Sanders PR, Flick JS, Adams SP, Bittner ML et al (1983) Expression of bacterial genes in plant cells. Proc Natl Acad Sci U S A 80(15):4803–4807 ArticleCASPubMedPubMed CentralGoogle Scholar
• Fuchs M, Gonsalves D (2007) Safety of virus resistant transgenic plants two decade after their introduction: lessons from realistic field risk assessment studies. Annu Rev Phytopathol 45:173–202 ArticleCASPubMedGoogle Scholar
• Fujisawa M, Takita E, Harada H, Sakurai N, Suzuki H, Ohyama K, Misawa N (2009) Pathway engineering of Brassica napus seeds using multiple key enzyme genes involved in ketocarotenoid formation. J Exp Bot 60(4):1319–1332 ArticleCASPubMedGoogle Scholar
• Gabard JM, Charest PJ, Iyer VN, Miki BL (1989) Cross-resistance to short residual sulfonylurea herbicides in transgenic tobacco plants. Plant Physiol 91(2):574–580 ArticleCASPubMedPubMed CentralGoogle Scholar
• Gaj T, Guo J, Kato Y et al (2012) Targeted gene knockout by direct delivery of zinc-finger nuclease proteins. Nat Methods 9:805–807 ArticleCASPubMedPubMed CentralGoogle Scholar
• Gaj T, Liu J, Anderson KE, Sirk SJ, Barbas CF (2013) Protein delivery using Cys2-His2 zinc-finger domains. ACS Chem Biol 9:1662–1667 ArticleGoogle Scholar
• Gasiunas G, Barrangou R, Horvath P, Siksnys V (2012) Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc Natl Acad Sci 109(39):E2579–E2586 ArticleCASPubMedPubMed CentralGoogle Scholar
• Gastélum-Estrada A, Serna-Saldívar SO, Jacobo-Velázquez DA (2021) Fighting the COVID-19 pandemic through biofortification: innovative approaches to improve the Immunomodulating capacity of foods. ACS Food Sci Technol 1(4):480–486 ArticleGoogle Scholar
• Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 551(7681):464–471 ArticleCASPubMedPubMed CentralGoogle Scholar
• Gbashi S, Oluwafemi A, Janet AA, Sarem T, Shandry T, Oluwaseun MA, Bunmi O, Julianah OO, Patrick N (2021) Food safety, food security and genetically modified organisms in Africa: a current perspective. Biotechnol Genet Eng Rev 37(1):30–63 ArticleCASPubMedGoogle Scholar
• George T, Graham PH, Roger H (2009) Genetically modified plants Google Scholar
• Gillespie S, Hodge J, Yosef S, Pandya-Lorch R (2016) Nourishing millions: stories of change in nutrition. Intl Food Policy Res Inst Google Scholar
• Gonsalves D (1998) Control of papaya ringspot virus in papaya: a case study. Annu Rev Phytopathol 36(1):415–437 ArticleCASPubMedGoogle Scholar
• Gonsalves D, Gonsalves C, Ferreira S, Pitz K, Fitch M, Manshardt R, Slightom J (2004) Transgenic virus resistant papaya: from hope to reality for controlling of papaya ringspot virus in Hawaii. APS net:1–12. feature story for July, 2004 Google Scholar
• Green JM, Castle LA (2010) Transitioning from single to multiple herbicide resistant crops. In: Nandula VK (ed) Glyphosate resistance in crops and weeds: history, development, and management. Wiley, Hoboken, NJ, pp 67–91 ChapterGoogle Scholar
• Griffiths AJF, Wessler SR, Lewontin RC, Gelbart WM, Suzuki DT, Miller JH (2005) Introduction to genetic analysis, 8th edn. W.H. Freeman, New York Google Scholar
• Gullner G, Kömives T, Rennenberg H (2001) Enhanced tolerance of transgenic poplar plants overexpressing gamma-glutamylcysteine synthetase towards chloroacetanilide herbicides. J Exp Bot 52(358):971–979 ArticleCASPubMedGoogle Scholar
• Guo J, Yang L, Liu X, Guan X, Jiang L, Zhang D (2009) Characterization of the exogenous insert and development of event-specific PCR detection methods for genetically modified Huanong no. 1 papaya. J Agric Food Chem 57:7205–7212 ArticleCASPubMedGoogle Scholar
• Gutterson N (2020) Commercialization and applications of agricultural biotechnology. In: Biotechnology entrepreneurship. Academic Press, pp 385–398 ChapterGoogle Scholar
• Haun W, Coffman A, Clasen BM, Demorest ZL, Lowy A, Ray E, Retterath A, Stoddard T, Juillerat A, Cedrone F, Mathis L, Voytas DF, Zhang F (2014) Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family. Plant Biotechnol J 12(7):934–940 ArticleCASPubMedGoogle Scholar
• Herrera-Estrella L, Ann Depicker MVM, Schell J (1983) Expression of Chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector. Nature 303(5914):209–213 ArticleCASGoogle Scholar
• Hilder VA, Gatehouse AM, Sheerman SE, Barker RF, Boulter D (1987) A novel mechanism of insect resistance engineered into tobacco. Nature 330(6144):160–163 ArticleCASGoogle Scholar
• Hilscher J, Bürstmayr H, Stoger E (2016) Targeted modification of plant genomes for precision crop breeding. Biotechnol J 12(1):1600173 ArticleGoogle Scholar
• Homrich MS, Wiebke-Strohm B, Weber RLM, Bodanese-Zanettini MH (2012) Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants. Genet Mol Biol 35(4):998–1010 ArticleCASPubMedPubMed CentralGoogle Scholar
• Huang G, Allen R, Davis EL, Baum TJ, Hussey RS (2006) Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene. Proc Natl Acad Sci 103(39):14302–14306 ArticleCASPubMedPubMed CentralGoogle Scholar
• Islam SMF, Karim Z (2019) World’s demand for food and water: the consequences of climate change. In: Farahani MHDA, Vatanpour V, Taheri AH (eds) Desalination–challenges and opportunities. IntechOpen Google Scholar
• Itoh N, Toda H, Matsuda M, Negishi T, Taniguchi T, Ohsawa N (2009) Involvement of S-adenosylmethionine-dependent halide/thiol methyltransferase (HTMT) in methyl halide emissions from agricultural plants: isolation and characterization of an HTMT-coding gene from Raphanus sativus (daikon radish). BMC Plant Biol 9(1):1–10 ArticleGoogle Scholar
• Jagdish T, Morris JJ, Wade BD, Blount ZD (2020) Probing the deep genetic basis of a novel trait in Escherichia coli. In: Evolution in action: past, present and future. Springer, Cham, pp 107–122 ChapterGoogle Scholar
• James C (1998) Global review of commercialized transgenic crops: the International Service for the Acquisition of Agri-biotech applications. ISAAA 8 Google Scholar
• Jansing J, Schiermeyer A, Schillberg S, Fischer R, Bortesi L (2019) Genome editing in agriculture: technical and practical considerations. Int J Mol Sci 20(12):2888 ArticleCASPubMedPubMed CentralGoogle Scholar
• Jiang WZ, Henry IM, Lynagh PG, Comai L, Cahoon EB, Weeks DP (2017) Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa, using CRISPR/Cas9 gene editing. Plant Biotechnol J 15(5):648–657 ArticleCASPubMedPubMed CentralGoogle Scholar
• Jones RA, Naidu RA (2019) Global dimensions of plant virus diseases: current status and future perspectives. Ann Rev Virol 6:387–409 ArticleCASGoogle Scholar
• Jones RA (2009) Plant virus emergence and evolution: origins, new encounter scenarios, factors driving emergence, effects of changing world conditions, and prospects for control. Virus Res 141(2):113–130 ArticleCASPubMedGoogle Scholar
• Jones RAC (2021) Global plant virus disease pandemics and epidemics. Plan Theory 10:233 CASGoogle Scholar
• Juma C (2011) Preventing hunger: biotechnology is key. Nature 479:471–472 ArticleCASPubMedGoogle Scholar
• Khan MS, Yu X, Kikuchi A, Asahina M, Watanabe KN (2009) Genetic engineering of glycine betaine biosynthesis to enhance abiotic stress tolerance in plants. Plant Biotechnol 26(1):125–134 ArticleCASGoogle Scholar
• Kim S, Takahashi M, Higuchi K, Tsunoda K, Nakanishi H, Yoshimura E, Nishizawa NK (2005) Increased nicotianamine biosynthesis confers enhanced tolerance of high levels of metals, in particular nickel, to plants. Plant Cell Physiol 46(11):1809–1818 ArticleCASPubMedGoogle Scholar
• Klas E, Fuchs M, Gonsalves D (2011) Fruit yield of virus-resistant transgenic summer squash in simulated commercial plantings under conditions of high disease pressure. J Hortic For 3(2):46–52 Google Scholar
• Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR (2016) Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature 533(7603):420–424 ArticleCASPubMedPubMed CentralGoogle Scholar
• Kumar K, Gambhir G, Dass A, Tripathi AK, Singh A, Jha AK et al (2020) Genetically modified crops: current status and future prospects. Planta 251:1–27 ArticleGoogle Scholar
• Kundu PALLOB, Mandal RK (2001) Transgenic approaches for producing Viru resistant plants. Proc-Ind Nat Sci Acad Part A 67(1/2):53–79 CASGoogle Scholar
• Labbé P, Jean-Philippe David H, Alout PM, Djogbenou L, Pasteur N, Weill M (2017) Evolution of resistance to insecticide in disease vectors. In: Genetics and evolution of infectious diseases: Second Edition, pp 313–339 ChapterGoogle Scholar
• Lamichhane JR, Reay-Jones FP (2021) Editorial: impacts of COVID-19 on global plant health and crop protection and the resulting effect on global food security and safety. Crop Protection (Guildford, Surrey) 139:105383 ArticleCASPubMedGoogle Scholar
• Layton MB, Williams MR, Stewart S (1997) BT-cotton in Mississippi: the first year. In: 1997 Proceedings Beltwide Cotton Conferences, vol 2. National Cotton Council, New Orleans, LA, USA, pp 861–863 Google Scholar
• Lermontova I, Grimm B (2000) Overexpression of Plastidic Protoporphyrinogen IX oxidase leads to resistance to the diphenyl-ether herbicide Acifluorfen. Plant Physiol 122(1):75–84 ArticleCASPubMedPubMed CentralGoogle Scholar
• Li T, Liu B, Spalding MH, Weeks DP, Yang B (2012) High-efficiency TALEN-based gene editing produces disease-resistant rice. Nat Biotechnol 30(5):390–392 ArticleCASPubMedGoogle Scholar
• Li M, Li X, Zhou Z, Wu P, Fang M, Pan X et al (2016) Reassessment of the four yield-related genes Gn1a, DEP1, GS3, and IPA1 in rice using a CRISPR/Cas9 system. Front Plant Sci 7:1–13 Google Scholar
• Li B, Clohisey SM, Chia BS et al (2020) Genome-wide CRISPR screen identifies host dependency factors for influenza A virus infection. Nat Commun 11:164. https://doi.org/10.1038/s41467-019-13965-xArticleCASPubMedPubMed CentralGoogle Scholar
• Littlejohn P, Finlay BB (2021) When a pandemic and an epidemic collide: COVID-19, gut microbiota, and the double burden of malnutrition. BMC Med 19:31 ArticleCASPubMedPubMed CentralGoogle Scholar
• Lobato-Gómez M, Hewitt S, Capell T, Christou P, Dhingra A, Girón-Calva PS (2021) Transgenic and genome-edited fruits: background, constraints, benefits, and commercial opportunities. Hortic Res 8 Google Scholar
• Lu Y, Tian Y, Shen R, Yao Q, Zhong D, Zhang X, Zhu JK (2021) Precise genome modification in tomato using an improved prime editing system. Plant Biotechnol J 19(3):415 ArticlePubMedGoogle Scholar
• Lucca P, Hurrell R, Potrykus I (2001) Genetic engineering approaches to improve the bioavailability and the level of iron in rice grains. Theor Appl Genet 102(2):392–397 ArticleCASGoogle Scholar
• Lutz KA, Knapp JE, Maliga P (2001) Expression of bar in the plastid genome confers herbicide resistance. Plant Physiol 125(4):1585–1590 ArticleCASPubMedPubMed CentralGoogle Scholar
• Macedo MLR, Oliveira CF, Oliveira CT (2015) Insecticidal activity of plant lectins and potential application in crop protection. Molecules 20(2):2014–2033 ArticlePubMedPubMed CentralGoogle Scholar
• Majeed H, Gillor O, Kerr B, Riley MA (2011) Competitive interactions in Escherichia coli populations: the role of bacteriocins. ISME J 5(1):71–81 ArticlePubMedGoogle Scholar
• Manosathiyadevan M, Bhuvaneshwari V, Latha R (2017) Impact of insects and pests in loss of crop production: a review. In: Sustainable agriculture towards food security, pp 57–67 ChapterGoogle Scholar
• Mansoor S, Amin I, Hussain M, Zafar Y, Briddon RW (2006) Engineering novel traits in plants through RNA interference. Trends Plant Sci 11(11):559–565 ArticleCASPubMedGoogle Scholar
• Mao YB, Cai WJ, Wang JW, Hong GJ, Tao XY, Wang LJ, Chen XY (2007) Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol. Nat Biotechnol 25(11):1307–1313 ArticleCASPubMedGoogle Scholar
• Martínez-Fortún J, Phillips DW, Jones HD (2017) Potential impact of genome editing in world agriculture. Emerg Top Life Sci 1(2):117–133 ArticlePubMedGoogle Scholar
• Martins-Salles S, Machado V, Massochin-Pinto L, Fiuza LM (2017) Genetically modified soybean expressing insecticidal protein (Cry1Ac): management risk and perspectives. Facets 2(1):496–512 ArticleGoogle Scholar
• Matveeva TV, Lutova LA (2014) Horizontal gene transfer from agrobacterium to plants. Front Plant Sci 5:326 ArticlePubMedPubMed CentralGoogle Scholar
• Meyer CJ, Norsworthy JK (2020) Timing and application rate for sequential applications of glufosinate are critical for maximizing control of annual weeds in LibertyLink® soybean. Int J Agron 2020:1ArticleGoogle Scholar
• Miao J, Guo D, Zhang J, Huang Q, Qin G, Zhang X et al (2013) Targeted mutagenesis in rice using CRISPR-Cas system. Cell Res 23:1233–1236 ArticleCASPubMedPubMed CentralGoogle Scholar
• Mohammed A, Abalaka ME (2011) Agrobacterium transformation: a boost to agricultural biotechnology. J Med Genet Genomics 3(8):126–130 CASGoogle Scholar
• Nadakuduti SS, Enciso-Rodríguez F (2021) Advances in genome editing with CRISPR systems and transformation technologies for plant DNA manipulation. Front Plant Sci 11:637159 ArticlePubMedPubMed CentralGoogle Scholar
• Nahar K, Hasanuzzaman M, Fujita M (2016) Roles of osmolytes in plant adaptation to drought and salinity. In: Osmolytes and plants acclimation to changing environment: emerging omics technologies. Springer, New Delhi, pp 37–68 ChapterGoogle Scholar
• Nicholas D, Rodríguez-Bravo B, Watkinson A, Boukacem-Zeghmouri C, Herman E, Xu J, Świgoń M (2017) Early career researchers and their publishing and authorship practices. Learned Publishing 30(3):205–217 ArticleGoogle Scholar
• Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A (2016) Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science 353(6305):aaf8729 ArticlePubMedGoogle Scholar
• Odipio J, Ogwok E, Taylor NJ, Halsey M, Bua A, Fauquet CM, Alicai T (2014) RNAi-derived field resistance to cassava brown streak disease persists across the vegetative cropping cycle. GM Crops Food 5(1):16–19 ArticlePubMedGoogle Scholar
• OECD, Woodhouse D (1999) Quality and quality assurance. Quality and internationalisation in higher education Google Scholar
• Ortigosa A, Gimenez-Ibanez S, Leonhardt N, Solano R (2019) Design of a bacterial speck resistant tomato by CRISPR/Cas9-mediated editing of SlJAZ2. Plant Biotechnol J 17:665–673 ArticleCASPubMedGoogle Scholar
• Osendarp S, Kweku Akuoku J, Black RE, Headey D, Ruel M, Scott N, Shekar M, Walker N, Flory A, Haddad L, Laborde D, Stegmuller A, Thomas M, Heidkamp R (2021) The COVID-19 crisis will exacerbate maternal and child undernutrition and child mortality in low- and middle-income countries. Nature Food 2:476–484 ArticleCASPubMedGoogle Scholar
• Padgette SR, Kolacz KH, Delannay X, Re DB, LaVallee BJ, Tinius CN, Rhodes KW, Otero YI, Barry GF, Eichholtz DA, Peschke VM, Nida DL, Taylor NB, Kishore GM (1995) Development, identification, and characterization of a glyphosate tolerant soybean line. Crop Sci 35(5):1451–1461 ArticleCASGoogle Scholar
• Parmar N, Singh KH, Sharma D, Singh L, Kumar P, Nanjundan J, Khan YJ, Chauhan DK, Thakur AK (2017) Genetic engineering strategies for biotic and abiotic stress tolerance and quality enhancement in horticultural crops: a comprehensive review. Biotech 7(4):239 Google Scholar
• Pausch P, Al-Shayeb B, Bisom-Rapp E, Tsuchida CA, Li Z, Cress BF et al (2020) CRISPR-Cas8 from huge phages is a hypercompact genome editor. Science 369:333–337 ArticleCASPubMedPubMed CentralGoogle Scholar
• Pérez-Massot E, Banakar R, Gómez-Galera S, Zorrilla-López U, Sanahuja G, Arjó G, Zhu C (2013) The contribution of transgenic plants to better health through improved nutrition: opportunities and constraints. Genes Nutr 8(1):29–41 ArticlePubMedGoogle Scholar
• Petersen C, Woods HA, Kingsolver JG (2000) Stage-specific effects of temperature and dietary protein on growth and survival of Manduca sexta caterpillars. Physiol Entomol 25:35–40 ArticleCASGoogle Scholar
• Petrova P, AbouRaya MM (2020) Global economic consequences and possible solutions to COVID-19 Великотърновски университет „Св. св. Кирил и Методий” 191–198 Google Scholar
• Pratiwi RA, Surya MI (2020) Agrobacterium-mediated transformation. In: Genetic transformation in crops. IntechOpen Google Scholar
• Qaim M, Kouser S (2013) Genetically modified crops and food security. PLoS One 8(6):e64879 ArticleCASPubMedPubMed CentralGoogle Scholar
• Quilis J, López-García B, Meynard D, Guiderdoni E, San Segundo B (2014) Inducible expression of a fusion gene encoding two proteinase inhibitors leads to insect and pathogen resistance in transgenic rice. Plant Biotechnol J 12(3):367–377 ArticleCASPubMedGoogle Scholar
• Rahangdale S, Singh Y, Katkani D, Surjaye N (2020) Chapter 2: Gene Transfer Methods in Plants. In: Gene transfer methods in plants. Integrated Publications, New Delhi Google Scholar
• Ravelonandro M, Scorza R, Bachelier JC, Labonne G, Levy L, Damsteegt V, Dunez J (1997) Resistance of transgenic Prunus domestica to plum pox virus infection. Plant Dis 81(11):1231–1235 ArticleCASPubMedGoogle Scholar
• Raymond Park J, McFarlane I, Hartley Phipps R, Ceddia G (2011) The role of transgenic crops in sustainable development. Plant Biotechnol J 9(1):2–21 ArticlePubMedGoogle Scholar
• Regina A, Bird A, Topping D, Bowden S, Freeman J, Barsby T, Morell M (2006) High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats. Proc Natl Acad Sci 103(10):3546–3551 ArticleCASPubMedPubMed CentralGoogle Scholar
• Rengasamy P (2005) World salinisation with emphasis on Australia. In: Comparative biochemistry and physiology a-molecular & integrative physiology. (141(3), p. S337-S337. Elsevier science Inc, 360 Park Ave South, New York, USA Google Scholar
• Ricroch A, Clairand P, Harwood W (2017) Use of CRISPR systems in plant genome editing: toward new opportunities in agriculture. Emerg Top Life Sci 1(2):169–182 ArticleCASPubMedPubMed CentralGoogle Scholar
• Rocha-Munive MG, Soberón M, Castañeda S, Niaves E, Scheinvar E, Eguiarte LE, Mota-Sánchez D, Rosales-Robles E, Nava-Camberos U, Martínez-Carrillo JL, Blanco CA, Bravo A, Souza V (2018) Evaluation of the impact of genetically modified cotton after 20 years of cultivation in Mexico. Front Bioeng Biotechnol 6:82 ArticlePubMedPubMed CentralGoogle Scholar
• Romeis J, Driesche RGV, Barratt BI, Bigler F (2008) Insect-resistant transgenic crops and biological control. In: Integration of insect-resistant genetically modified crops within IPM programs. Springer, Dordrecht, pp 87–117 ChapterGoogle Scholar
• Römer P, Hahn S, Jordan T, Strauß T, Bonas U, Lahaye T (2007) Plant pathogen recognition mediated by promoter activation of the pepper Bs3 resistance gene. Science 318(5850):645–648 ArticlePubMedGoogle Scholar
• Safari F, Zare K, Negahdaripour M, Barekati-Mowahed M, Ghasemi Y (2019) CRISPR Cpf1 proteins: structure, function and implications for genome editing. Cell Biosci 9:36. https://doi.org/10.1186/s13578-019-0298-7. PMID: 31086658; PMCID: PMC6507119 ArticlePubMedPubMed CentralGoogle Scholar
• Scholes J, Endacott R, Biro M, Bulle B, Cooper S, Miles M (2012) Clinical decision-making: midwifery students' recognition of, and response to, post-partum haemorrhage in the simulation environment. BMC Pregnancy Childbirth 12:19 ArticlePubMedPubMed CentralGoogle Scholar
• Schornack S, Meyer A, Römer P, Jordan T, Lahaye T (2006) Gene-for-gene-mediated recognition of nuclear-targeted AvrBs3-like bacterial effector proteins. J Plant Physiol 163(3):256–272 ArticleCASPubMedGoogle Scholar
• Schütte G, Eckerstorfer M, Rastelli V, Reichenbecher W, Restrepo-Vassalli S, Ruohonen-Lehto M, Wuest Saucy A, Mertens M (2017) Herbicide resistance and biodiversity: agronomic and environmental aspects of genetically modified herbicide-resistant plants. Environ Sci Eur 29:5 ArticlePubMedPubMed CentralGoogle Scholar
• Shan Q, Voytas DF (2018) Editing plant genes one base at a time. Nat Plants 4:412–413 ArticleCASPubMedGoogle Scholar
• Shelton AM, Olmstead DL, Burkness EC, Hutchison WD, Dively G, Welty C, Sparks AN (2013) Multi-state trials of Bt sweet corn varieties for control of the corn earworm (Lepidoptera: Noctuidae). J Econ Entomol 106(5):2151–2159 ArticleCASPubMedGoogle Scholar
• Shelton AM, Hossain MJ, Paranjape V, Azad AK, Rahman ML, Khan ASMMR, Prodhan MZH, Rashid MA, Majumder R, Hossain MA, Hussain SS, Huesing JE, McCandless L (2018) Bt eggplant project in Bangladesh: history, present status, and future direction. Front Bioeng Biotechnol 3(6):106 ArticleGoogle Scholar
• Shimatani Z, Kashojiya S, Takayama M, Terada R, Arazoe T, Ishii H, Teramura H, Yamamoto T, Komatsu H, Miura K, Ezura H, Nishida K, Ariizumi T, Kondo A (2017) Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol 35(5):441–443 ArticleCASPubMedGoogle Scholar
• Shukla VK, Doyon Y, Miller JC, DeKelver RC, Moehle EA, Worden SE, Mitchell JC, Arnold NL, Gopalan S, Meng X, Choi VM, Rock JM, Wu YY, Katibah GE, Zhifang G, McCaskill D, Simpson MA, Blakeslee B, Greenwalt SA, Butler HJ, Urnov FD (2009) Precise genome modification in the crop species Zea mays using zinc-finger nucleases. Nature 459(7245):437–441 ArticleCASPubMedGoogle Scholar
• Siminszky B, Corbin FT, Ward ER, Fleischmann TJ, Dewey RE (1999) Expression of a soybean cytochrome P450 monooxygenase cDNA in yeast and tobacco enhances the metabolism of phenylurea herbicides. Proc Natl Acad Sci U S A 96(4):1750–1755 ArticleCASPubMedPubMed CentralGoogle Scholar
• Singh M, Kumar J, Singh S, Singh VP, Prasad SM (2015) Roles of osmoprotectants in improving salinity and drought tolerance in plants: a review. Rev Environ Sci Biotechnol 14(3):407–426 ArticleCASGoogle Scholar
• Singla J, Krattinger SG (2016) Biotic stress resistance genes in wheat. In: Wrigley C, Corke H, Seetharaman K, Faubion J (eds) Encyclopedia of food grains (Second Edition). Academic Press, pp 388–392 ChapterGoogle Scholar
• Sinha S, Sandhu K, Bisht N, Naliwal T, Saini I, Kaushik P (2019) Ascertaining the paradigm of secondary metabolism enhancement through gene level modification in therapeutic plants. J Young Pharm 11(4):337 ArticleCASGoogle Scholar
• Soltani N, Shropshire C, Sikkema PH (2020) Weed control in Dicamba-resistant soybean with glyphosate/Dicamba applied at various doses and timings. Int J Agron Google Scholar
• Song A, Zhu X, Chen F, Gao H, Jiang J, Chen S (2014) A chrysanthemum heat shock protein confers tolerance to abiotic stress. Int J Mol Sci 15(3):5063–5078 ArticlePubMedPubMed CentralGoogle Scholar
• Subramanyam K, Sailaja KV, Subramanyam K, Muralidhara Rao D, Lakshmidevi K (2011) Ectopic expression of an osmotin gene leads to enhanced salt tolerance in transgenic chilli pepper (Capsicum annum L.). Plant Cell Tissue Organ Cult (PCTOC) 105(2):181–192 ArticleCASGoogle Scholar
• Sun YW, Jiao GA, Liu ZP, Zhang X, Li JY, Guo XP, Du WM, Du JL, Francis F, Zhao YD, Xia LQ (2017) Generation of high-amylose rice through CRISPR/Cas9-mediated targeted mutagenesis of starch branching enzymes. Front Plant Sci 8:298. https://doi.org/10.3389/fpls.2017.00298ArticlePubMedPubMed CentralGoogle Scholar
• Sunilkumar G, Campbell LM, Puckhaber L, Stipanovic RD, Rathore KS (2006) Engineering cottonseed for use in human nutrition by tissue-specific reduction of toxic gossypol. Proc Natl Acad Sci 103(48):18054–18059 ArticleCASPubMedPubMed CentralGoogle Scholar
• Suzuki Y, Makino A, Mae T (2001) Changes in the turnover of rubisco and levels of mRNAs of rbcL and rbcS in rice leaves from emergence to senescence. Plant Cell Environ 24(12):1353–1360 ArticleCASGoogle Scholar
• Symington LS, Gautier J (2011) Double-strand break end resection and repair pathway choice. Annu Rev Genet 45:247–271 ArticleCASPubMedGoogle Scholar
• Taheri F, Azadi H, D’Haese M (2017) A world without hunger: organic or GM crops? Sustainability 9(4):580 ArticleGoogle Scholar
• Takabe T, Nakamura T, Nomura M, Hayashi Y, Ishitani M, Muramoto Y, Tanaka A (1998) Glycinebetaine and the genetic engineering of salinity tolerance in plants. In: Stress responses of photosynthetic organisms: molecular mechanisms and molecular regulations. Elsevier Science, Amsterdam, pp 115–131 ChapterGoogle Scholar
• Tanaka H, Yabuta Y, Tamoi M, Tanabe N, Shigeoka S (2015) Generation of transgenic tobacco plants with enhanced tocotrienol levels through the ectopic expression of rice homogentisate geranylgeranyl transferase. Plant Biotechnol 32:233–238 ArticleCASGoogle Scholar
• Tang GL, Galili G, Zhuang X (2007) RNAi and microRNA: breakthrough technologies for the improvement of plant nutritional value and metabolic engineering. Metabolomics 3:357–369 ArticleCASGoogle Scholar
• Tien P, Wu G (1991) Satellite RNA for the biocontrol of plant disease. Adv Virus Res 39:321–339 ArticleCASPubMedGoogle Scholar
• Tohidfar M, Khosravi S (2015) Transgenic crops with an improved resistance to biotic stresses. A review. Biotechnol Agron Soc Environ 19:62–70 Google Scholar
• Tripathi S, Suzuki JY, Ferreira SA, Gonsalves D (2008) Papaya ringspot virus-P: characteristics, pathogenicity, sequence variability and control. Mol Plant Pathol 9(3):269–280 ArticleCASPubMedPubMed CentralGoogle Scholar
• UNCTAD (2017) The role of science, technology and innovation in ensuring food security by 2030. United Nations, New York and Geneva Google Scholar
• Vasconcelos M, Datta K, Oliva N, Khalekuzzaman M, Torrizo L, Krishnan S, Datta SK (2003) Enhanced iron and zinc accumulation in transgenic rice with the ferritin gene. Plant Sci 164(3):371–378 ArticleCASGoogle Scholar
• Vincelli P (2016) Genetic engineering and sustainable crop disease management: opportunities for case-by-case decision-making. Sustainability 8(5):495 ArticleGoogle Scholar
• Waltz E (2014) Beating the heat. Nat Biotechnol 32:610–613 ArticleCASPubMedGoogle Scholar
• Wang F, Wang C, Liu P, Lei C, Hao W, Gao Y, Liu YG, Zhao K (2016) Enhanced Rice blast resistance by CRISPR/Cas9-targeted mutagenesis of the ERF transcription factor gene OsERF922. PLoS One 11(4):e0154027 ArticlePubMedPubMed CentralGoogle Scholar
• Wang L, Samac DA, Shapir N, Wackett LP, Vance CP, Olszewski NE, Sadowsky MJ (2005) Biodegradation of atrazine in transgenic plants expressing a modified bacterial atrazine chlorohydrolase (atzA) gene. Plant Biotechnol J 3(5):475–486 ArticleCASPubMedGoogle Scholar
• Wang Y, Cheng X, Shan Q, Zhang Y, Liu J, Gao C, Qiu JL (2014) Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nat Biotechnol 32(9):947–951 ArticleCASPubMedGoogle Scholar
• Welch RM, Graham RD (2004) Breeding for micronutrients in staple food crops from a human nutrition perspective. J Exp Bot 55(396):353–364 ArticleCASPubMedGoogle Scholar
• Wesseler J, Purnhagen K (2020) Is the Covid-19 pandemic a game changer in GMO regulation? EuroChoices 19(3):49–52 ArticleGoogle Scholar
• Wirth J, Poletti S, Aeschlimann B, Yakandawala N, Drosse B, Osorio S, Sautter C (2009) Rice endosperm iron biofortification by targeted and synergistic action of nicotianamine synthase and ferritin. Plant Biotechnol J 7(7):631–644 ArticleCASPubMedGoogle Scholar
• Wisniewski M, Fuchigami L, Wang Y, Srinivasan C, Norilli J (2002) Overexpression of a cytosolic ascorbate peroxidase gene in apple improves resistance to heat stress. In: XXXVI International horticultural congress and exhibition, p 147 Google Scholar
• Yang Y, Al-Khayri JM, Anderson EJ (1997) Transgenic spinach plants expressing the coat protein of cucumber mosaic virus. In Vitro Cellular & Developmental Biology-Plant 33(3):200–204 ArticleCASGoogle Scholar
• Zafar K, Sedeek KEM, Rao GS, Khan MZ, Amin I, Kamel R et al (2020) Genome editing Technologies for Rice Improvement: Progress, prospects, and safety concerns. Front Genome Ed 2:1–16 ArticleGoogle Scholar
• Zha S, Boboila C, Alt FW (2009) Mre11: roles in DNA repair beyond homologous recombination. Nat Struct Mol Biol 16(8):798–800 ArticleCASPubMedGoogle Scholar
• Zhang HX, Zhang Y, Yin H (2019) Genome editing with mRNA encoding ZFN, TALEN, and Cas9. Mol Ther 27:735–746 ArticleCASPubMedPubMed CentralGoogle Scholar
• Zhang Y, Bai Y, Wu G, Zou S, Chen Y, Gao C, Tang D (2017) Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat. Plant J 91(4):714–724 ArticleCASPubMedGoogle Scholar
• Zhang Y, Li D, Zhang D, Zhao X, Cao X, Dong L et al (2018a) Analysis of the functions of TaGW2 homoeologs in wheat grain weight and protein content traits. Plant J 94:857–866 ArticleCASPubMedGoogle Scholar
• Zhang Y, Liang Z, Zong Y, Wang Y, Liu J, Chen K et al (2016) Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA. Nat Commun 7:1–8 Google Scholar
• Zhang Y, Massel K, Godwin ID et al (2018b) Applications and potential of genome editing in crop improvement. Genome Biol 19:210 ArticleCASPubMedPubMed CentralGoogle Scholar
• Zhou J, Peng Z, Long J, Sosso D, Liu B, Eom JS et al (2015) Gene targeting by the TAL effector PthXo2 reveals cryptic resistance gene for bacterial blight of rice. Plant J 82:632–643 ArticleCASPubMedGoogle Scholar
• Zhu YX, Ou-Yang WJ, Zhang YF, Chen ZL (1996) Transgenic sweet pepper plants from agrobacterium mediated transformation. Plant Cell Rep 16(1):71–77 ArticleCASPubMedGoogle Scholar
• Zilberman D, Holland TG, Trilnick I (2018) Agricultural GMOs—what we know and where scientists disagree. Sustainability 10(5):1514 ArticleGoogle Scholar

URLs

• Brookes G (2020) Crop biotechnology continues to provide higher farmer income and significant environmental benefits. Available at https://pgeconomics.co.uk/press+releases/25/Crop+biotechnology+continues+to+provide+higher+farmer+income+and+significant+environmental+benefits
• DiLeo M (2012) Monsanto’s GM drought tolerant corn. Available at https://biofortified.org/2012/08/monsantos-gm-drought-tolerant-corn/
• DuPont Pioneer. Announces Intentions to Commercialize First CRISPR-Cas Product. Press Release (2016). Available at https://www.pioneer.com/home/site/about/news-media/news-releases/template.CONTENT/guid.1DB8FB71-1117-9A56-E0B6-3EA6F85AAE92
• FAO (2019) New standards to curb the global spread of plant pests and diseases. Available at https://www.fao.org/news/story/en/item/1187738/icode/#:~:text=FAO%20estimates%20that%20annually%20between,insects%20around%20US%2470%20billion
• ISAAA (2018) Global status of commercialized biotech/GM crops in 2018: biotech crops continue to help meet the challenges of increased population and climate change. ISAAA Brief No.54. ISAAA, Ithaca, NY. Available at https://www.isaaa.org/resources/publications/briefs/54/executivesummary/default.aspGoogle Scholar
• ISAAA (2019a) Executive summary, ISAAA Brief 55-2019: global status of commercialized biotech/GM crops: 2019 Google Scholar
• ISAAA (2019b) Biotech crop highlights in 2019. ISAAA Publication Pocket K No. 16. ISAAA: Ithaca, NY. Available at https://www.isaaa.org/resources/publications/pocketk/16/
• Johnson YD, O’Connor S (2015) These charts show every genetically modified food people already eat in the U.S. TIMES newsletter. https://time.com/3840073/gmo-food-charts/
• The World Bank Report (2021) Learning losses from COVID-19 could cost this generation of students close to $17 trillion un lifetime earnings. https://www.worldbank.org/en/news/press-release/2021/12/06/learning-losses-from-covid-19-could-cost-this-generation-ofstudents-close-to-17-trillion-in-lifetime-earnings